With larger particles, it takes a long time for the correlation of the signal to decay, whereas the more rapid movement of smaller particles means the correlation of the signal decreases more quickly. The fluctuation is measured by comparing the signal intensity at one time with signal intensity a very short time later. The intensity fluctuates more rapidly with small particles than it does with large particles. The speed that particles diffuse at as a result of Brownian motion is calculated by measuring the rate of fluctuation in the intensity of the scattered light. Researchers can learn about the interactions between particles in the fluid, as well as other aspects such as thermal melting points. Analysis over time can also provide information about changes in the solution. This is done by measuring the rate at which the intensity of the scattered light fluctuates when detected using a suitable optical arrangement. The light scattering that occurs when a light is shone onto particles in solution can provide useful information about the size, weight, surface structure, charge, stability and concentration of the particles. 3 DLS technical note MRK656-01 How DLS Works In dynamic light scattering, the speed at which the particles are diffusing due to Brownian motion is measured. In DLS, light scattering by the particles is used to monitor Brownian motion. The solvent molecules have more impact on the movement of smaller p articles, which therefore move more rapidly. Multispeckle DLS (DLS with CCD) Using CCD as an area detector instead of a photomultiplier and replacing time averaging partly by ensemble averaging to shorten sampling time and improve the statistical accuracy Apollo P.Y. Particles suspended in a liquid medium undergo Brownian motion, which is slower, the larger the particle is. The size of liposomes varies, but is typically around 100nm. DLS can help to provide accurate information about the size of liposomes, which is critical to ensuring that drug delivery systems are effective. Liposome size and the amount of drug that can be loaded into liposomes is key in terms of the drugsвЂ™ pharmacodynamic and pharmacokinetic parameters. Liposomes are mainly used in the biopharmaceutical industry as carriers for drug delivery in cancer treatment. The technique can be used to determine the size of liposomes, spherical bilayer vesicles that may be naturally derived or synthesized from man-made substances. The technique is also referred to as photon correlation spectroscopy and quasi-elastic light scattering. The DLS particle size analyzer has the advantages of accuracy, rapidity, and good repeatability for the measurement of Nano particles, emulsions or suspensions. Dynamic light scattering (DLS) is a technique that is used to measure the size of particles, generally particles that are in the sub-micron region. Dynamic light scattering (DLS), also known as photon correlation spectroscopy (PCS), is a commonly used characterization method for nanoparticles.

Dynamic light scattering dls